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FT-NIR spectroscopy and RP-HPLC combined with multivariate analysis reveals differences in plant cell suspension cultures of Thevetia peruviana treated with salicylic acid and methyl jasmonate

dc.contributor.authorMendoza, Dary
dc.contributor.otherArias, Juan Pablo
dc.contributor.otherCuaspud, Olmedo
dc.contributor.otherRuiz, Orlando
dc.contributor.otherArias, Mario
dc.date.accessioned2022-11-15T19:44:55Z
dc.date.available2022-11-15T19:44:55Z
dc.date.issued2020-08-10
dc.date.submitted2020-01-31
dc.identifier.urihttps://hdl.handle.net/20.500.12834/851
dc.description.abstractPlant cell suspension culture of T. peruviana is a feasible biotechnological platform for the production of secondary metabolites with anti-proliferative/cytotoxic activity, as phenolic compounds (PC); however, different in in vitro growth conditions may affect the production, demanding strategies to increase the metabolite biosynthesis, as well as the development of sensitive and rapid analytical methods for metabolite monitoring. The Fourier transform near-infrared (FT-NIR) spectroscopy and Reversed-phase high-performance liquid chromatography (RP-HPLC) combined with Multivariate analysis (MVA) were used to detect significant differences in the PC production in cultures treated with two elicitors. The results suggest that the FT-NIR-MVA is useful for discriminating samples according to the treatment, showed significant influence of the PC signal. RP-HPLC-MVA showed that the elicitor effect occurs at 72 h post-elicitation. Detection of dihydroquercetin (maximum concentration = 12.59 mg/L), a flavonoid with anti-cancer properties, is highlighted. Future studies will be aimed at scaling this culture to increase the productivity of dihydroquercetin.spa
dc.format.mimetypeapplication/pdfspa
dc.language.isoengspa
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/*
dc.sourceBiotechnology Reportsspa
dc.titleFT-NIR spectroscopy and RP-HPLC combined with multivariate analysis reveals differences in plant cell suspension cultures of Thevetia peruviana treated with salicylic acid and methyl jasmonatespa
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dc.audiencePúblico generalspa
dc.identifier.doi10.1016/j.btre.2020.e00519
dc.identifier.instnameUniversidad del Atlánticospa
dc.identifier.reponameRepositorio Universidad del Atlánticospa
dc.rights.ccAttribution-NonCommercial 4.0 International*
dc.subject.keywordsThevetia peruviana Plant cell culture FT-NIR RP-HPLC Multivariate analysisspa
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